Then, the absorbance value at 450 nm was determined using a microplate reader (SpectraMAX 340; Molecular Devices, Sunnyvale, CA, United States). With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. 312 gain calibration failed. 313 reference gain check fail. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The SpectraMax 384 Plus lets you get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. The plates were incubated for 4 additional hours at 37°C. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hIranian Journal of Basic Medical Sciences ijbms. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. Donor filter: 616/10 nm. 3 mm. 0. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. Since the formation of NADPH is stoichiometrically related to the oxidation of glucose-6-phosphate, measurements for glucose + fructose can be determined by the absorbance of NADPH at 340 nm. HTRF-compatible SpectraMax Multi-Mode Readers. Assay setup for a 384-well low-volume plate. Microplate Spectrophotometers. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that. 5. 2. Cell Assay Cells (5 × 10 5 per well) are plated in triplicate in 96-well plates in 0. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. The Spectra Catalina 340 Automated Connect 12Volt or 24Volt Catalina 340C produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize. Pharmacokinetic and pharmacodynamic analysis. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Created Date: 20051213090753Zmeasured using a SpectraMax 340 multiwell plate. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. The slope of the linear portion of the kinetic curve generated from each well was used to. ). The optical density at 405 nm (OD 405) of the plates was measured with a microplate reader (SpectraMax 340 PC384, Molecular Devices) before and after a 1-h incubation at room temperature. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 1. , Sunnyvale, CA, USA) at 595 nm. Photometric Range: 0. 024). For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Clotting times (mean ± SD) for rSimukunin and rSV. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. 4) was followed according to Vanacker et al. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. After adding sample, the mixture was incubated for 30 min at 37°C, with one unit of enzymatic activity being defined as an increase in absorbance of 0. SpectraMAX 340 plate reader (Molecular Devices). 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Figure 1. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. 4) was followed according to Vanacker et al. 2. (1988) and recorded at 412 nm. 5), L-ascorbic acid (25. Awareness Technology, Inc. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. One plate carrier adapter is provided with the instrument. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 4 0112-0126D SafetyInformation. SpectraMax 190 reads 96-well and strip-well plates. Growth was monitored at 30°C every 6 min for 8 to 12 h at 660 nm using a multiscan photometer (spectraMax 340; Molecular Devices). measurements were performed on a SPECTRAmax-340 plate reader (Molecular Devices Inc. Bertin Precellys Evolution Touch Homogenizer. FlexStation® 3 Multi-Mode Microplate Reader. Briefly, cells were seeded into a. Excitation 1 340 nm Emission 1 520 nm Cut off for emission 1 495 nm Excitation 2 340 nm Emission 2 620 nm Cut off for emission 2 570 nm Sensitivity Readings 50 to 100 PMT Optimal Column Wavelenght Column Priority . Lactate Dehydrogenase (LDH) Release. 190–850 nm <± 1. Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. Example: [Drawer]. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Values are expressed as the mean s ± SD of 3 separate experiments, each performed in triplicate. 7. Quick View. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. An absorbance was read at 490/630 nm using a spectraMAX 340 microtiter plate reader. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. SpectraMax Gemini EM & FLEXstation (bottom read) 1. Driessen*,MaaikeA. 00 USD. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. A 0. Wavelength(s) 340 to 850 nm; Add to Compare List. Molecular Devices Corporation 1311. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). Details. the SpectraMax iD3 is a fantastic plate reader for a. 2 0. used in the SPECTRAmax PLUS. PerkinElmer. The cell proliferation on different Ti discs was assessed by measuring the absorbance at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Devices, Sunnyvale, CA). Stat Fax 3200 Microplate Reader. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. CD154 is an established indicator of antigen-reactive T helper cells in other species, but has not been characterized in horses. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise results. 0 to 4. The membranes were. SpectraMax Multi-Mode Microplate Readers. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- pathways and protein activation and expression in one system. 7 macrophages were obtained from ATCC (Manassas, VA). The spectrophotometers used were SpectraMax 340 (for GSH, GCS, and HNE-His assays) and SpectraMax M3 (for GGT, GPx, and GST assays), both from Molecular Devices, Sunnyvale, CA. Rate of fibrinolysis. ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. 0 0 The indicated microplates were scanned form 200 nm to 800 nm. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). 1% N-1-naphtylethylenediamine dihydrochloride, 2. Here, we show how the SpectraMax® i3x and SpectraMax® iD5 Multi-Mode Microplate Readers are used to perform robust, no-wash saturation binding assays using HTRF. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. 5. The mixture was centrifuged for 10 min at 10,000 rpm, and BSA concentration in the supernatant was determined using bicinchoninic acid (BCA) assay. Samples were considered “positive” if the absorbance value was greater than two times the average absorbance value of the negative control wells . At that point halfway up the maximum transmittance value, the total width (nm) of the transmitted band is. Other technologies such as imaging. Details. About The SpectraMax GEMINI EM is controlled by an external computer running SoftMax ® Pro software which provides integrated instrument control, data display, and statistical data analysis. TDP-43 stress granular cells were treated with 70% ice-cold. using SpectraMax i3 (Molecular devices, San Jose, USA). SpectraMAX 340. Bioz Stars score: 86/100, based on 1 PubMed citations. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). Studies of the regulation of AoSMC migration by Angpt2 were preformed using a wound healing assay. 305 reference level saturation. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. . Near bottom. Binding affinity for denosumab. well, and strip-well microplates. Money Saved: $340. NO release assayThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. The SpectraTest validation package provides a NIST-traceable solution for validating optical performance of the SpectraMax Plus 384 reader and does it automatically. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. 0, MSD) or Spectramax 340 (Molecular Devices). The first time you replace an OEM lamp you must use the N-34-MLA. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. 5. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. Six wells were assayed for each set of conditions and stadard deviations. NHDFs were seeded in microtiter plates (96 wells) at a density of 1x105 cells per well. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance plate reader (Molecular Devices, Union City, CA). The possi-ble effect of zinc or copper on the binding of heme by A was tested as described above by using a 50 and 100 M final concentration of each metal. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Unit has several areas of damaged plastic. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. Number of flashes. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Fully tested with Warranty. 5 ng/ml; estradiol: 10 pg/ml. Molecular Devices SpectraMax Gemini XS Microplate Reader. SpectraMax Microplate Readers. Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. The SpectraMax® Gemini™ EM Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. The contents of the. Microplate Readers. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. SpectraMax Multi-Mode Microplate Readers. Emission. The plates were read using an ELISA microplate reader (Molecular Devices SPECTRAMAX 340 PC 384, Sunnyvale, California, USA) at 450 nm. SpectraMAX 340. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- plates can be used in the SPECTRAmax PLUS 384. ir Neuroprotection of a sesamin derivative, 1, 2-bis [(3-methoxy- phenyl) methyl] ethane-1, 2-dicaroxylic acid (MMEDA) againstEach SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. Integration delay. MSD read buffer and TMB one component HRP substrate are from MSD and SurModics (Eden Prairile, MN), respectively. 5 mL sample or standard solution was well mixed with 0. See More Microplate Reader / Microtiter Plate Reader. TDP-43 stress granular cells were treated with 70% ice-cold. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Page: 1/6. On 1, 7, 14, and 21 days, the cells were washed with DPBS twice and incubated with CCK-8 solution for 2 h in the dark at 37 °C. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. VersaMax reads 96-well and strip-well plates. Data were expressed as the mean percent viable cell vs. #N207) LAL Reagent Water (Lonza cat. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. All assays were performed in triplicate, and the absorbance at 405 nm was measured using a SpectraMax 340 multiwell plate reader (Molecular Devices). Both readers feature an 8-channel read head for fast reads and. Screening for Antigen-Specific IgG Producing Clones by Capture ELISA. 2. Absorbance was read at 450 nm using a Molecular Device Spectramax 340. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). The extreme flexibility and high sensitivity of the SpectraMax GEMINI EM make it appropriate for applications within the fields of bio-A microplate reader that can detect two or more applications is considered a multi-mode plate reader. P. gp70 blocking. Plasma glucose levels were measured using FreeStyle Lite (Abbott Diabetes Care Ltd. 303 unable to cal dark current. Molecular Devices SpectraMax M5e Microplate Reader. Includes: Computer Loaded with Softmax Pro 5. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. Novak, Nathan J. Multi-mode Microplate Readers. 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. edu Contact Phone: 216-368-8962. The mixture changed to a red color by a reaction of LDH that was measured by a plate reader (SpectraMax 340 PC; Molecular Devices, San Jose, CA, USA) at a visible wavelength of. Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. Samples containing 25 μg of protein were separated on 12. Data were expressed as the mean LPS-treated cells (100%) vs. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. MCE has not independently confirmed the accuracy of these methods. Awareness Technology Inc. Molecular Device SpectraMax M3 Plate Reader - Certified with Warranty. Incubation with TMB substrate was done for 18 min. Polystyrene plates (384-well) were precoated overnight at 4°C with 2 μg/ml of murine, human, or chimeric RANKL. Customer Transformation Hub. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. , Fremont, CA, USA). Not all manufacturers' microplates are the same with regard to design, materials, orIn a typical 100-μL assay with mixtures containing 50 mM MOPS, pH 6. Wound healing assay. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects andSPECTRAmax ® PLUS 384 Microplate Spectrophotometer. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. , Sunnyvale, CA). Thin layer chromatography (TLC) was. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Features showcased by this model include. 2 - 3. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. The absorbance was measured at 550 nm with a Spectramax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hFollowing incubation, absorbance was immediately measured at 405 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, LLC. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). . 0% and ± 0. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. control. 20. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 0 3. SpectraMax® Microplate Readers and SoftMax® Pro Software. The absorbance at 450 nm was determined using a microplate reader (spectraMAX 340). #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Spectral Range. 30 µs. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. About GMI provides new and GMI Certified Pre-Owned scientific instrumentation and service to research universities, environmental labs, forensic investigators, biotechnology and pharmaceutical companies, hospitals, clinics and analytical laboratories, in the United. chen@case. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). , Sunnyvale, Calif. control or celatrol-treated cells. 1% Chaps, 10 m M dithiothreitol, and 10% sucrose. The loading equaled the mass of BSA per mass of particles. Inter-assay variation was prevented by testing all of the samples on the same day. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. Molecular Devices SPECTRAmax. Summary of Contents for Molecular Devices SPECTRAmax 340PC. Samples with OD values of less than 0. #N207) LAL Reagent Water (Lonza cat. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature. 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. This assay measures the two major sugars in wine, glucose and fructose. Subsequently, the mixture was heated at 100 °C for 10. The absorbance values were measured at 450 nm using a SpectraMax® 340 plate reader (Molecular Devices, Sunnyvale, CA, USA). A 340 μL of 50 mM borate buffer (pH 9), 240 μL methanol, 10 μL standard solution or sample, and 20 μL of 0. TGFα release was calculated as described in the Results. The inhibition percentage was determined by linear regression through ranges of 20 to 30. The cell survival. Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. Or scan up to 384 wells at any time to determine the best assay conditions. Near bottom. Glutathione reductase (GR) activity was measured, according to Smith et al. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. 5. 190-1000 nm. The. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. Despite the relevance of adaptive immunity against equine pathogens antigen-specific T cell responses of horses are not well characterized and the lack of insight into T cell responses hampers the understanding of the pathogeneses of important diseases. Related Products: SpectraMax 340 Absorbance Microplate Reader, Molecular Devices SpectraMax 384 Plus UV/Vis Microplate Reader, Molecular DevicesGETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS applications guide to microplate systems applications guide to microplate systems450 nm using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Anti-proteolytic activity. Molecular Devices Corporation 1311. 2. #N207) LAL Reagent Water (Lonza cat. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). 5% phosphoric acid) in microplates at room temperature for 10 min. Manager: Yinghua Chen, PhD Contact Email: yinghua. SpectraMax Multi-Mode Microplate Readers. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). 2. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). G. Absorbance was measured at 490/650 nm by using a SpectraMax 340 microplate spectrophotometer® (Molecular Devices, Sunnyvale, CA, U. Methods: We investigated growth inhibition by varying concentrations of AMB in seven isolates of C. using SpectraMax i3 (Molecular devices, San Jose, USA). 1 All Necessary Cables and Cords Manuals General Specifications Dimensions (in. The light features a slim profile to prevent. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. 4; 100 m M NaCl, 0. 003 OD Stray light ≤ 0. 6 (h) x 22. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. Cytotoxicity was determined by measuring the release of LDH. 5. Product Keyword. SoftMax Pro. Donor: 616/10 nm. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. Versteegh, Yoran H. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. TheSpectraMax®Plus384addstheabilitytoreadcuvettes. 190-1000 nm, tunable in 1 nm increments. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. We also offer depot Repair Service from our base of operations in Southern California (San Diego). #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. 5), L-ascorbic acid (25. To correct for inter. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 314 low lamp level warning. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. Data were expressed as the mean percent viable cell vs. , Sunnyvale, CA). These lab workhorses are used in drug discovery, research, bioassay validation, and. As a common indicator of equine T cell activation, cytokine induction. PC12 or BV-2 cells treated with various concentrations of BBD were stressed with hypoxia for one hour and the supernatant was then assayed for LDH activity. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. 2. Read one sample or up to 384. 5mM), and changes in OD 405 nm. 30. Immunofluorescence and Aggregation Analysis. Microplate Spectrophotometer Operator’s Manual. Molecular Device M2e. using SpectraMax i3 (Molecular devices, San Jose, USA). Unit powers. After washing, the dye was eluted with 3% HCl, and the absorbance measured with a SpectraMax 340 microtiter plates reader at 665 nm. The concentration of NO in the cells was determined by measuring the amount of nitrite, a relatively stable oxidation product of. 1. The plate was read on a SpectraMax 340 PC plate reader (Molecular Devices, Sunnyvale, CA) at 450 nm, with reference at 650 nm. The protein concentration was determined spectrophotometrically at 280 nm in a 1 cm quartz cuvette using a SPECTRAmax-340 UV–vis spectrophotometer. The sensitivities of the hormones are Prolactin: 0. 00 USD. ) at 490 nm. Details. , Sunnyvale CA). FACS analysis. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. Not all manufacturers' microplates are the same with regard to design, materials, orAbsorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Assay buffer was 50 m M Hepes, pH 7. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 340-850 nm: Wavelength Selection: Monochromator, turnable in 1 nm increments:. 304 signal level saturation. Microplate Readers. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). The growth. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. The fluorescence intensity was measured using the SpectraMax 340 microplate reader (Molecular Devices) at the following conditions: excitation at 475 nm and emission at 529 nm (green fluorescence) or 590 nm (red fluorescence).